, for instance, demonstrates an amperometric stream mobile. Effluent from your column passes about the working electrode—held at a relentless potential relative to some downstream reference electrode—that completely oxidizes or decreases the analytes.
각각 다른 산업 분야에 대한 자세한 정보 및 다양한 카테고리는 다음 써모 피셔 사이언티픽 학습 센터에서 산업 및 응용 과학 페이지를 확인하세요.
Column issues: A soiled or destroyed column can result in peak broadening. Contaminants can accumulate around the column as time passes, hindering analyte separation. Often thoroughly clean the column based on the producer's Guidance. If cleaning does not aid, consider changing the column.
By following the following pointers and systematically addressing prospective will cause, you may efficiently troubleshoot frequent HPLC difficulties and make certain your analyses are accurate and trusted.
物質にエネルギーを与える（励起）ことにより発光する（蛍光）性質を利用した検出器。一般に選択性が高く高感度で、物質に特異的な検出が可能。蛍光する性質を持たない物質については、その物質を標識することにより検出が可能になる。
Fluoxetine is an additional identify for your antidepressant drug Prozac. The perseverance of fluoxetine in serum is an important Element of monitoring its therapeutic use.
-hydroxybenzoic acid (PH) on the nonpolar C18 column matter to your utmost analysis time of six min. The shaded areas represent regions exactly where a separation is not possible, With all the unresolved solutes recognized.
. Block diagram of the click here HPLC–MS. A 3 component mixture enters the HPLC. When ingredient A elutes with the column, it enters the MS ion supply and ionizes to form the parent ion and a number of other fragment ions.
four. Should the peaks for fluoxetine and protriptyline are settled insufficiently, how might you alter the cell phase to boost their separation?
-hydroxybenzoic acid (PH) with a nonpolar C18 column topic to your optimum Assessment time of 6 min. The shaded regions represent areas where by a separation is not possible, With all the unresolved solutes determined.
Conversely, a flow charge that may be as well minimal could potentially cause excessive band broadening. Examine your stream level settings and modify them in accordance with the proven technique.
If your cellular click here section’s pH is sufficiently acidic, the solutes are existing as neutral weak acids that happen to be far more soluble within the stationary section and choose extended to elute. Because the weak acid solutes do not need equivalent p
Cellular phase impurities: Contaminants during the mobile phase can elute within the column and clearly show up as ghost peaks. Prepare a new mobile period with high-purity solvents and take into consideration filtering the cell section prior to use.
To result a better separation concerning two solutes we must Increase the selectivity element, (alpha). There's two widespread techniques for expanding (alpha): incorporating a reagent towards the cell phase that reacts While using the solutes within a secondary equilibrium response or switching to a special cellular phase.